Shiraz University of Medical Sciences

Thermostable chitinase from Cohnella sp. A01: isolation and product optimization
Abstract :
Twelve bacterial strains isolated from shrimp farming ponds were screened for their growth activity on chitin as the sole carbon source. The highly chitinolytic bacterial strain was detected by qualitative cup plate assay and tentatively identified to be Cohnella sp. A01 based on 16S rDNA sequencing and by matching the key morphological, physiological, and biochemical characteristics. The cultivation of Cohnella sp. A01 in the suitable liquid medium resulted in the production of high levels of enzyme. The colloidal chitin, peptone, and K2HPO4 represented the best carbon, nitrogen, and phosphorus sources, respectively. Enzyme production by Cohnella sp. A01 was optimized by the Taguchi method. Our results demonstrated that inoculation amount and temperature of incubation were the most significant factors influencing chitinase production. From the tested values, the best pH/temperature was obtained at pH 5 and 70°C, with Km and Vmax values of chitinase to be 5.6mg/mL and 0.87μmol/min, respectively. Ag+, Co2+, iodoacetamide, and iodoacetic acid inhibited the enzyme activity, whereas Mn2+, Cu2+, Tweens (20 and 80), Triton X-100, and EDTA increased the same. In addition, the study of the morphological alteration of chitin treated by enzyme by SEM revealed cracks and pores on the chitin surface, indicating a potential application of this enzyme in several industries.

 

 

 AUTHORS :
Nasrin Aliabadi – Shiraz University of Medical Sciences
Saeed Aminzadeh – National Institute of Genetic Engineering and Biotechnology
Ali Asghar Karkhane – National Institute of Genetic Engineering and Biotechnology
Kamahldin Haghbeen – National Institute of Genetic Engineering and Biotechnology

 

Shiraz Second International Congress on Mobile Health

Mobile Health or mHealth,as a sub-segment of eHealth,is a term used for the practice of medicine and public health supported by mobile devices.The main goal of the congress is to provide a vehicle for exchanging ideas,concepts,techniques,experiments and applications in using mobile communication devices,such as mobile phones,tablet computers and handheld PCs,for health services and information.Shiraz International mHealth congress-2017 mainly focuses on the delivery of healthcare services via mobile communication and multimedia technologies.Authors are kindly requested to submit their abstract via the website of the congress. Accepted abstracts will be published on the congress official website and in the supplement of the Shiraz E-Medical Journal (this journal is currently indexed/abstracted in some major indexing systems such as Scopus.).

Website: http://mhealth.sums.ac.ir/en/

Congress to be held on :  February 22-23 2017
Address: Shiraz Information Technology Incubator of Medical Sciences – Next to Sina & Sadra Convention Halls – Neshat street – Shiraz – Iran